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plasmid pcmv flag yap s381a  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pcmv flag yap s381a
    Plasmid Pcmv Flag Yap S381a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s381a/pCMV-Flag-YAP-S381A+(Plasmid+%2333090)/pm40694420-236-8-10
    Average 93 stars, based on 2 article reviews
    plasmid pcmv flag yap s381a - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Identification of PKN2 and MOB4 as Coordinators of Collective Cell Migration
    Article Snippet: .. The pQCXIH-Myc-YAP-5SA plasmid encoding constitutively active Myc-tagged YAP1 (Uniprot entry P46937-2) comprising the 5 serine to alanine substitutions S61A, S109A, S127A, S164A, and S381A was a gift from Kun-Liang Guan (Addgene plasmid #33093). .. To obtain stable cell lines, cells were transfected with Lipofectamine 3000 (ThermoFisher Scientific), then diluted and selected with either 1 μg/ml of Puromycin (PKN2 and MOB4) or 15 μg/ml of Hygromycin (YAP1).

    Article Title: Hippo pathway mediates resistance to cytotoxic drugs
    Article Snippet: Pancreatic cancer cell lines Panc1, Panc02.13, BcPC3, Miapaca2, Panc10.05, Capan2, YAPC, CFPAC1, PATU-8902, PATU-8988S, DANG, and ASPC1 cells and mesothelioma cell line H2052 were obtained from American Type Culture Collection. .. YAP expression construct with serine-to-alanine mutations at S61A, S109A, S127A, S128A, S131A, S163A, S164A, and S381A was purchased from Addgene (plasmid ID: 42562). .. GIPZ Lentiviral shRNAmir clones for human YAP1 or NF2 were purchased from Dharmacon.

    Expressing:

    Article Title: Hippo pathway mediates resistance to cytotoxic drugs
    Article Snippet: Pancreatic cancer cell lines Panc1, Panc02.13, BcPC3, Miapaca2, Panc10.05, Capan2, YAPC, CFPAC1, PATU-8902, PATU-8988S, DANG, and ASPC1 cells and mesothelioma cell line H2052 were obtained from American Type Culture Collection. .. YAP expression construct with serine-to-alanine mutations at S61A, S109A, S127A, S128A, S131A, S163A, S164A, and S381A was purchased from Addgene (plasmid ID: 42562). .. GIPZ Lentiviral shRNAmir clones for human YAP1 or NF2 were purchased from Dharmacon.

    Construct:

    Article Title: Hippo pathway mediates resistance to cytotoxic drugs
    Article Snippet: Pancreatic cancer cell lines Panc1, Panc02.13, BcPC3, Miapaca2, Panc10.05, Capan2, YAPC, CFPAC1, PATU-8902, PATU-8988S, DANG, and ASPC1 cells and mesothelioma cell line H2052 were obtained from American Type Culture Collection. .. YAP expression construct with serine-to-alanine mutations at S61A, S109A, S127A, S128A, S131A, S163A, S164A, and S381A was purchased from Addgene (plasmid ID: 42562). .. GIPZ Lentiviral shRNAmir clones for human YAP1 or NF2 were purchased from Dharmacon.



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